Benzothiazole-Containing Tetrazolium Salts That Produce Water-Soluble Formazan Dyes Absorbing at a Long Wavelength upon NADH Reduction.
نویسندگان
چکیده
منابع مشابه
WST-1 Reduction at the RBC Plasma Membrane
Tetrazolium dyes have often been used as electron acceptors in measurements of cell proliferation rates (Berridge and Tan, 1993; Ishiyama, et al., 1993; Berridge, et al., 1996), in histochemical procedures (Zamudio, et al., 1969; May, et al., 1995b; Demehin, et al., 2001), and for assaying redox enzymes (Berridge and Tan, 1993; Mittler and Zilinskas, 1993; Debnam and Shearer, 1997; Ye, et al., ...
متن کاملReduction of tetrazolium salts by pleuropneumonialike organisms.
Tetrazolium salts have proved useful as indicators of electron transfer. Compounds such as triphenyltetrazolium chloride' are more practical than indicators like methylene blue since they are converted from a soluble colorless salt to an insoluble formazan in the reduced state. They also provide similar convenience in detecting enzymatic activity and specific sites of such activity (Bielig, Kau...
متن کاملSpectrophotometric Assay for Superoxide Dismutase Based on the Reduction of Highly Water-soluble Tetrazolium Salts by Xanthine-Xanthine Oxidase.
Two novel highly water-soluble tetrazolium salts, WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt) and WST-8 (4-[3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt) were applied to the assay of superoxide dismutase (SOD). The superoxide anion generated by xanthine/xanthine oxidase (XO) reduced WST-...
متن کاملDifferential Effect of Tetrazolium Dyes upon Bacteriophage Plaque Assay Titers.
This study examined whether the practice of incorporating either tetrazolium red or tetrazolium violet dye into plaque assay medium deleteriously influences plaque assay titers. Representative members of six different virus families were studied: Cystoviridae (varphi6), Leviviridae (MS2), Microviridae (varphiX174), Myoviridae (T2), Podoviridae (P22), and Siphoviridae (Denver, T1, and VD13). Eac...
متن کاملWater soluble salts of fredericamycin A: preparation and biological activity.
logical^ properties of fredericamycin A (FM-A, NSC-305263) a novel antitumor antibiotic produced by Streptomyces griseus (FCRC-48) were reported earlier. The structure (1) was elucidated by spectroscopic and X-ray techniques1'3'4) followed by its biosynthesis,5) electron spin resonance,6) and synthetic studies.n FM-Aexhibited good in vitro activity against Gram-positive bacteria, fungi and was ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Sciences
سال: 1996
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.12.515